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Monday, 3 February 2014

DNA of peanut-allergic kids changes with immune therapy

Peanut allergy, like other food allergies, currently has no cure. Scientists are conducting clinical trials of doctor-supervised immunotherapy, in which peanut-allergic patients take increasing amounts of peanut powder to try to desensitize them to the peanut allergen. At the end of the trial, patients are usually asked to eat some peanuts every day for the rest of their lives.

The gradual introduction of small doses of peanuts to people with a peanut allergy could alter how their DNA reacts, according to new research from Stanford University and Packard Children’s Hospital. The research supports a growing body of evidence for oral immunotherapy (OIT) including recent positive results from the University of Cambridge. This latest study, published in the journal The Lancet, suggests that when kids slowly increase their tolerance to the allergen, OIT changes how their genes express themselves inside the body.

"At first, eating two peanut butter cups a day might seem fun, but it gets a little boring and a lot of people might stop," said Kari Nadeau, MD, PhD, associate professor of pediatrics at Stanford and an immunologist at Stanford Hospital & Clinics and Lucile Packard Children's Hospital Stanford. Until now, doctors could not test whether patients who had completed immunotherapy could safely stop eating daily doses of peanuts, she said. "Our new finding can help us try to determine whether, for the long term, someone's allergy has truly been shut off so people can eat ad lib."

Nadeau is the senior author of a paper describing the new findings, which will be published online Jan. 31 in the Journal of Allergy and Clinical Immunology.

Currently, doctors have no cure for peanut allergies, which can cause severe reactions including death. In the study, researchers examined the blood cells of a group of 20 peanut-allergic children and adults who had undergone immunotherapy for two years. That meant that doctors had these patients eat gradually increasing amounts of peanut powder every day for two years until they became desensitized to the allergen. At the start of the trial, the patients were already able to eat four grams of peanuts every day with no signs of an allergic reaction.

To test the success of the immunotherapy (and find out the mechanisms behind it) the doctors told the patients to stop eating peanuts for three months, and then gave them a small dose. Seven out of the 20 patients had no reaction to the peanuts, while 13 regained their allergy. The researchers compared the white blood cells of the patients who could now tolerate peanuts with a control group who had never had immunotherapy before. More specifically, the researchers looked at the genes within these white blood cells.

For the patients who never received immunotherapy (the control group), a large number of chemical tags called methyl groups were now affixed to their DNA. These methyl groups controlled how certain genes actively expressed themselves in the body. The large clump found on the control group’s genes had locked, or silenced, the genes that were supposed to help them fight off the allergic reactions.

In contrast, the researchers found that the seven patients who could now tolerate peanuts showed a very sparse number of methyl groups on their DNA, meaning that the genes that help them control their allergic reactions were free to actively work within their body.

The research was funded by Food Allergy Research & Education, the Fund for Food Allergy Research at Stanford, the National Institute of Allergy and Infectious Diseases (grant R211R21AI09583801) and the Children’s Health Research Institute/Lucile Packard Foundation for Children’s Health. The research also was supported by the National Center for Research Resources (grant UL1RR024128) and the National Center for Advancing Translational Science (grant UL1TR000093).

Source :
http://esciencenews.com/articles/2014/02/03/dna.peanut.allergic.kids.changes.with.immune.therapy.stanfordpackard.study.finds
http://med.stanford.edu/ism/2014/january/peanut.html
http://www.medicaldaily.com/peanut-allergy-oral-immunotherapy-changes-dna-alteration-gene-expression-possible-way-monitor-268328

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